topflash luciferase reporter assay Search Results


m50 super 8x topflash reporter plasmid  (Promega)
90
Promega m50 super 8x topflash reporter plasmid
M50 Super 8x Topflash Reporter Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+luciferase+reporter+assay/super+8x+topflash+luciferase+reporter/pmc06945285-108-12-27
Average 90 stars, based on 1 article reviews
m50 super 8x topflash reporter plasmid - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
topflash and fopflash luciferase reporter  (Promega)
90
Promega topflash and fopflash luciferase reporter
Sfrp1 inhibits Wnt7a activity in the <t>TOPflash</t> luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 <t>and</t> <t>pcDNA3.1-Dkk1</t> , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.
Topflash And Fopflash Luciferase Reporter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+luciferase+reporter+assay/topflash+and+fopflash+luciferase+reporter/pmc06056652-69-14-19
Average 90 stars, based on 1 article reviews
topflash and fopflash luciferase reporter - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
tcf luciferase reporter constructs topflash  (Promega)
90
Promega tcf luciferase reporter constructs topflash
Sfrp1 inhibits Wnt7a activity in the <t>TOPflash</t> luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 <t>and</t> <t>pcDNA3.1-Dkk1</t> , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.
Tcf Luciferase Reporter Constructs Topflash, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+luciferase+reporter+assay/tcf+luciferase+reporter+constructs+topflash/pmc05740794-43-7-11
Average 90 stars, based on 1 article reviews
tcf luciferase reporter constructs topflash - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
luciferase reporter (topflash)  (MicroSource Discovery Systems)
90
MicroSource Discovery Systems luciferase reporter (topflash)
A, Position frequency matrix of the T‐cell factor (TCF) motif was obtained from the JASPAR database ( http://jaspar.genereg.net ). B, <t>TOPFlash</t> consists of tandemly repeated TCF motifs (Wnt response elements [WREs]) upstream of a minimal promoter that drives <t>luciferase</t> gene expression. FOPFlash has mutated motifs (mWREs) and is used to normalize the TOPFlash activity. HAL reporter was developed as a luciferase reporter driven by 8 copies of the promoter of histidine ammonia‐lyase ( HAL ). Transcription factor (TF) that regulates the activity of HAL promoter is under investigation. C, These reporter plasmids were designed for monitoring the activity of Wnt/β‐catenin pathway in cultured cells. When the pathway is inhibited, TOPFlash and HAL reporter activities are decreased and increased, respectively
Luciferase Reporter (Topflash), supplied by MicroSource Discovery Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+luciferase+reporter+assay/luciferase+reporter++topflash+/pmc07060471-15-2-15
Average 90 stars, based on 1 article reviews
luciferase reporter (topflash) - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
topflash luciferase reporter construct  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare topflash luciferase reporter construct
A, Position frequency matrix of the T‐cell factor (TCF) motif was obtained from the JASPAR database ( http://jaspar.genereg.net ). B, <t>TOPFlash</t> consists of tandemly repeated TCF motifs (Wnt response elements [WREs]) upstream of a minimal promoter that drives <t>luciferase</t> gene expression. FOPFlash has mutated motifs (mWREs) and is used to normalize the TOPFlash activity. HAL reporter was developed as a luciferase reporter driven by 8 copies of the promoter of histidine ammonia‐lyase ( HAL ). Transcription factor (TF) that regulates the activity of HAL promoter is under investigation. C, These reporter plasmids were designed for monitoring the activity of Wnt/β‐catenin pathway in cultured cells. When the pathway is inhibited, TOPFlash and HAL reporter activities are decreased and increased, respectively
Topflash Luciferase Reporter Construct, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+luciferase+reporter+assay/topflash+luciferase+reporter+construct/10__1158_slash_1535___7163__mct___11___0873-93-8-19
Average 90 stars, based on 1 article reviews
topflash luciferase reporter construct - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
b-catenin luciferase reporter m50 super 8 topflash  (Promega)
90
Promega b-catenin luciferase reporter m50 super 8 topflash
A, Position frequency matrix of the T‐cell factor (TCF) motif was obtained from the JASPAR database ( http://jaspar.genereg.net ). B, <t>TOPFlash</t> consists of tandemly repeated TCF motifs (Wnt response elements [WREs]) upstream of a minimal promoter that drives <t>luciferase</t> gene expression. FOPFlash has mutated motifs (mWREs) and is used to normalize the TOPFlash activity. HAL reporter was developed as a luciferase reporter driven by 8 copies of the promoter of histidine ammonia‐lyase ( HAL ). Transcription factor (TF) that regulates the activity of HAL promoter is under investigation. C, These reporter plasmids were designed for monitoring the activity of Wnt/β‐catenin pathway in cultured cells. When the pathway is inhibited, TOPFlash and HAL reporter activities are decreased and increased, respectively
B Catenin Luciferase Reporter M50 Super 8 Topflash, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+luciferase+reporter+assay/b+catenin+luciferase+reporter+m50+super+8+topflash/pm23523860-96-9-32
Average 90 stars, based on 1 article reviews
b-catenin luciferase reporter m50 super 8 topflash - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
nfκb luciferase reporters  (System Biosciences Inc)
86
System Biosciences Inc nfκb luciferase reporters
A, Position frequency matrix of the T‐cell factor (TCF) motif was obtained from the JASPAR database ( http://jaspar.genereg.net ). B, <t>TOPFlash</t> consists of tandemly repeated TCF motifs (Wnt response elements [WREs]) upstream of a minimal promoter that drives <t>luciferase</t> gene expression. FOPFlash has mutated motifs (mWREs) and is used to normalize the TOPFlash activity. HAL reporter was developed as a luciferase reporter driven by 8 copies of the promoter of histidine ammonia‐lyase ( HAL ). Transcription factor (TF) that regulates the activity of HAL promoter is under investigation. C, These reporter plasmids were designed for monitoring the activity of Wnt/β‐catenin pathway in cultured cells. When the pathway is inhibited, TOPFlash and HAL reporter activities are decreased and increased, respectively
Nfκb Luciferase Reporters, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+luciferase+reporter+assay/expressing+lentiviruses+luciferase+reporters+topflash/pmc12445586-95-14-27
Average 86 stars, based on 1 article reviews
nfκb luciferase reporters - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier
topflash fopflash luciferase reporters  (Upstate Biotechnology Inc)
86
Upstate Biotechnology Inc topflash fopflash luciferase reporters
A, Position frequency matrix of the T‐cell factor (TCF) motif was obtained from the JASPAR database ( http://jaspar.genereg.net ). B, <t>TOPFlash</t> consists of tandemly repeated TCF motifs (Wnt response elements [WREs]) upstream of a minimal promoter that drives <t>luciferase</t> gene expression. FOPFlash has mutated motifs (mWREs) and is used to normalize the TOPFlash activity. HAL reporter was developed as a luciferase reporter driven by 8 copies of the promoter of histidine ammonia‐lyase ( HAL ). Transcription factor (TF) that regulates the activity of HAL promoter is under investigation. C, These reporter plasmids were designed for monitoring the activity of Wnt/β‐catenin pathway in cultured cells. When the pathway is inhibited, TOPFlash and HAL reporter activities are decreased and increased, respectively
Topflash Fopflash Luciferase Reporters, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+luciferase+reporter+assay/fopflash+luciferase+reporters+topflash/10__1074_slash_jbc__m901248200-37-0-8
Average 86 stars, based on 1 article reviews
topflash fopflash luciferase reporters - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier
topflash firefly luciferase reporter plasmid m50  (Adagene Inc)
86
Adagene Inc topflash firefly luciferase reporter plasmid m50
A, Position frequency matrix of the T‐cell factor (TCF) motif was obtained from the JASPAR database ( http://jaspar.genereg.net ). B, <t>TOPFlash</t> consists of tandemly repeated TCF motifs (Wnt response elements [WREs]) upstream of a minimal promoter that drives <t>luciferase</t> gene expression. FOPFlash has mutated motifs (mWREs) and is used to normalize the TOPFlash activity. HAL reporter was developed as a luciferase reporter driven by 8 copies of the promoter of histidine ammonia‐lyase ( HAL ). Transcription factor (TF) that regulates the activity of HAL promoter is under investigation. C, These reporter plasmids were designed for monitoring the activity of Wnt/β‐catenin pathway in cultured cells. When the pathway is inhibited, TOPFlash and HAL reporter activities are decreased and increased, respectively
Topflash Firefly Luciferase Reporter Plasmid M50, supplied by Adagene Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+luciferase+reporter+assay/firefly+luciferase+m50+plasmid+reporter+topflash/us12502369-1138-7-15
Average 86 stars, based on 1 article reviews
topflash firefly luciferase reporter plasmid m50 - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

Image Search Results


Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.

Journal: Frontiers in Molecular Neuroscience

Article Title: Opposite Roles of Wnt7a and Sfrp1 in Modulating Proper Development of Neural Progenitors in the Mouse Cerebral Cortex

doi: 10.3389/fnmol.2018.00247

Figure Lengend Snippet: Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.

Article Snippet: The Sfrp1 , Dkk1 coding sequences were subcloned into the pcDNA3.1 vector for the TOPflash and FOPflash luciferase reporter (Promega, United States) assay.

Techniques: Activity Assay, Luciferase, Reporter Assay, Activation Assay, Binding Assay, Expressing, Control, Plasmid Preparation, Mutagenesis, Transfection, Comparison

A, Position frequency matrix of the T‐cell factor (TCF) motif was obtained from the JASPAR database ( http://jaspar.genereg.net ). B, TOPFlash consists of tandemly repeated TCF motifs (Wnt response elements [WREs]) upstream of a minimal promoter that drives luciferase gene expression. FOPFlash has mutated motifs (mWREs) and is used to normalize the TOPFlash activity. HAL reporter was developed as a luciferase reporter driven by 8 copies of the promoter of histidine ammonia‐lyase ( HAL ). Transcription factor (TF) that regulates the activity of HAL promoter is under investigation. C, These reporter plasmids were designed for monitoring the activity of Wnt/β‐catenin pathway in cultured cells. When the pathway is inhibited, TOPFlash and HAL reporter activities are decreased and increased, respectively

Journal: Cancer Science

Article Title: Discovery of chemical probes that suppress Wnt/β‐catenin signaling through high‐throughput screening

doi: 10.1111/cas.14297

Figure Lengend Snippet: A, Position frequency matrix of the T‐cell factor (TCF) motif was obtained from the JASPAR database ( http://jaspar.genereg.net ). B, TOPFlash consists of tandemly repeated TCF motifs (Wnt response elements [WREs]) upstream of a minimal promoter that drives luciferase gene expression. FOPFlash has mutated motifs (mWREs) and is used to normalize the TOPFlash activity. HAL reporter was developed as a luciferase reporter driven by 8 copies of the promoter of histidine ammonia‐lyase ( HAL ). Transcription factor (TF) that regulates the activity of HAL promoter is under investigation. C, These reporter plasmids were designed for monitoring the activity of Wnt/β‐catenin pathway in cultured cells. When the pathway is inhibited, TOPFlash and HAL reporter activities are decreased and increased, respectively

Article Snippet: Hexachlorophene , Luciferase reporter (TOPFlash) , HEK293 expressing hFz‐1 (Wnt3a‐CM) , 960 (Genesis Plus Collection, MicroSource Discovery) , β‐Catenin degradation through SIAH‐1 induction , MWT = 406.89 CLog P = 7.02708 H‐bond donors = 2 H‐bond acceptors = 2 , , .

Techniques: Luciferase, Gene Expression, Activity Assay, Cell Culture

Inhibitors of Wnt/β‐catenin signaling discovered by high‐throughput screening (HTS)

Journal: Cancer Science

Article Title: Discovery of chemical probes that suppress Wnt/β‐catenin signaling through high‐throughput screening

doi: 10.1111/cas.14297

Figure Lengend Snippet: Inhibitors of Wnt/β‐catenin signaling discovered by high‐throughput screening (HTS)

Article Snippet: Hexachlorophene , Luciferase reporter (TOPFlash) , HEK293 expressing hFz‐1 (Wnt3a‐CM) , 960 (Genesis Plus Collection, MicroSource Discovery) , β‐Catenin degradation through SIAH‐1 induction , MWT = 406.89 CLog P = 7.02708 H‐bond donors = 2 H‐bond acceptors = 2 , , .

Techniques: HTS Assay, Luciferase, Inhibition, Fluorescence, Imaging, Drug discovery, Activation Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Over Expression, Amplified Luminescent Proximity Homogenous Assay, Expressing, Binding Assay